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OwnerVsevolod V. Belousov, Sergey A. Lukyanov +1inventors
InventorsVsevolod V. Belousov, Sergey A. Lukyanov, Arkady F. Fradkov
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The present invention provides a novel genetically encoded fluorescent indicator of hydrogen peroxide. The indicator comprises a sensor polypeptide which is responsive to hydrogen peroxide and a circularly permuted fluorescent protein which is operatively inserted into the sensor polypeptide. Interaction of the sensor polypeptide with hydrogen peroxide results in a change in fluorescence of the fluorescent protein. An isolated nucleic acid molecule encoding a hydrogen peroxide fluorescent indicator of the present invention is also provided. Also provided are vectors, expression cassettes, host-cells, stable cell lines, transgenic animals and transgenic plants comprising a nucleic acid of the present invention. Also provided are methods that use a fluorescent protein of the present invention or the nucleic acid encoding it.
A genetically engineered fluorescent indicator of hydrogen peroxide, comprising a sensor polypeptide which is responsive to hydrogen peroxide and a circularly permuted fluorescent protein which is operatively inserted into a flexible region of the sensor polypeptide and wherein the spectral properties of the circularly permuted fluorescent protein are affected by the responsiveness of the sensor polypeptide to hydrogen peroxide.
A genetically engineered nucleic acid molecule comprising a sequence that encodes a fluorescent indicator of hydrogen peroxide, wherein the fluorescent indicator comprises a sensor polypeptide which is responsive to hydrogen peroxide and a circularly permuted fluorescent protein which is operatively inserted into a flexible region of the sensor polypeptide through linker polypeptide moieties, and wherein the fluorescence of the fluorescent protein is affected by the responsiveness of the sensor polypeptide to hydrogen peroxide.
Dependencies are inferred from the claim text.