Cameras for fluorescence and reflectance imaging

A fluorescence imaging video system configured for acquiring color and multi-channel fluorescence/reflectance images, including:

• a single multi-mode light source for producing both multi-wavelength excitation light for fluorescence imaging and illumination light having red, green and blue components,
• a plurality of light source filters positionable between the light source and an illumination optical transmission system, each of the filters positioned stationarily during an imaging mode and transmitting substantially all the multi-wavelength excitation light intensity and selectively transmitting a predetermined portion of one or more of the red, green and blue component intensity;
• the optical transmission system directing the filtered light to a tissue sample and an imaging optical transmission system collecting reflected light and multi-wavelength fluorescence light produced by the tissue;
• a camera receiving the light collected by the optical transmission system, the camera including:
• a first color image sensor having a first spectral filter positioned in front of the color image sensor for selectively blocking the multi-wavelength excitation light and transmitting reflectance light at wavelengths other than the multi-wavelength excitation light, and
• a second color image sensor having a second spectral filter positioned in front of the color image sensor for selectively blocking the multi-wavelength excitation light and transmitting multi-wavelength fluorescence light at wavelengths other than the multi-wavelength excitation light.