Automated Parallel Oligonucleotide Synthesis
OwnerDavid Evansinventor
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Abstract
The invention is a system and method for the automated synthesis of biopolymers, such as polynucleotides. The synthesis reactions are conducted asynchronously in separate reaction volumes to minimize idle times for the machinery and to allow shorter polynucleotides to be removed when complete, rather than at the end of the entire run.
Claims32
A method for the automated synthesis of polynucleotides comprising the steps of:
- a) conducting a synthetic sequence comprising a repeating plurality of reactions in a first reaction volume, wherein each reaction is triggered by injecting reagents into the first reaction volume with an injection device and the reactions produce a polynucleotide;
- and
- b) conducting a synthetic sequence comprising a repeating plurality of reactions in a second reaction volume, wherein each reaction is triggered by injecting reagents into the second reaction volume with an injection device and the reactions produce a polynucleotide;
- wherein the synthetic sequence in the first reaction volume is asynchronous with the second synthetic sequence in the second reaction volume.
A system for the automated synthesis of polynucleotides, comprising a first reaction volume and a second reaction volume, an injection device having injectors for delivering reagents into the first reaction volume and the second reaction volume;
- an xy table configured to movably position the first reaction volume and the second reaction volume adjacent the injection device, and a controller configured to conduct a repeating plurality of reactions corresponding to a synthetic sequence in the first reaction volume and the second reaction volume, wherein the synthetic sequence in the first reaction volume is asynchronous with the synthetic sequence in the second reaction volume.
Dependencies are inferred from the claim text.