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US 2008/0177048 A1Published application
Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
AssigneeBio-Rad Laboratories, Inc.
InventorPeter S. Gagnon
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Abstract
This invention relates to the use of mixed mode chromatography for purification of at least one intact non-aggregated antibody from a mixture containing intact non-aggregated antibodies and undesirable materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies suitable for in vivo applications.
Claims27
A method for enhancing the antibody binding capacity of a mixed mode chromatography support, the method comprising
- contacting said support with an antibody preparation in the presence of an aqueous-soluble nonionic organic polymer.
Dependencies are inferred from the claim text.