Protein forming complex with c-Fos protein, nucleic acid encoding the same and method of using the same
AssigneeKeio University
InventorsEtsuko Miyamoto, Masamichi Ishizaka, Hiroshi Yanagawa
Life of this patent
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Abstract
Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.
Claims4
1-132. (canceled)
A method for inhibiting formation of a complex between a target protein that interacts with a c-Fos protein and the c-Fos protein, said method comprising:
- introducing a protein into a system containing the c-Fos protein and in which the complex is to be formed, wherein said protein is selected from the group consisting of:
- (a) the protein comprising the amino acid sequence of SEQ ID NO:
- 96;
- (b) the protein comprising the amino acid sequence of SEQ ID NO:
- 97;
- and
- (c) the protein comprising the amino acid sequence of SEQ ID NO:
- 96, including a deletion, substitution or addition of one amino acid residue, and which interacts with the c-Fos protein;
- and
- (d) the protein comprising the amino acid sequence of SEQ ID NO:
- 97, including a deletion, substitution or addition of one amino acid residue, and which interacts with the c-Fos protein.
A method for inhibiting formation of a complex between a target protein that interacts with a c-Fos protein and the c-Fos protein, said method comprising:
- introducing a protein into a system containing the c-Fos protein and in which the complex is to be formed, wherein said protein is selected from the group consisting of:
- (i) the protein encoded by the nucleotide sequence of SEQ ID NO:
- 160, and which interacts with the c-Fos protein;
- (ii) the protein encoded by the nucleotide sequence of SEQ ID NO:
- 161, and which interacts with the c-Fos protein;
- (iii) the protein encoded by the nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequence of SEQ ID NO:
- 160, and which interacts with the c-Fos protein, wherein said stringent conditions comprise a wash in 0.1×SSC, 0.1% SDS for 15 minutes at 60° C.;
- and
- (iv) the protein encoded by the nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequence of SEQ ID NO:
- 161, and which interacts with the c-Fos protein, wherein said stringent conditions comprise a wash in 0.1×SSC, 0.1% SDS for 15 minutes at 60° C.
Dependencies are inferred from the claim text.