Components of the presenilin-complex
AssigneeCellzome Limited
InventorsRichard HALE, Adele ROWLEY
Life of this patent
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Abstract
The present invention is not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and accompanying figures. Such modifications are intended to fall within the scope of the appended claims. Various publications are cited herein, the disclosures of which are incorporated by reference in their entireties.
Claims49
A protein complex comprising
- (a) a first protein, or a functionally active fragment or functionally active derivative thereof, which first protein is selected from the group consisting of:
- (i) Sambiasin-1 (SEQ ID No:
- 1) or a functionally active derivative thereof, or a functionally active fragment thereof, or a homolog thereof, or a variant of Sambiasin-1 encoded by a nucleic acid that hybridizes to the Sambiasin-1 nucleic acid or its complement under low stringency conditions,
- (b) and at least one second protein, or a functionally active fragment or functionally active derivative thereof, which second protein is selected from the group consisting of:
- (i) Presenilin-1 (SEQ ID No:
- 2), or a functionally active derivative thereof, or a functionally active fragment thereof, or a homolog thereof, or a variant of Presenilin-1 encoded by a nucleic acid that hybridizes to the Presenilin-1 nucleic acid or its complement under low stringency conditions,
- (ii) Nicastrin (SEQ ID No:
- 3), or a functionally active derivative thereof, or a functionally active fragment thereof, or a homolog thereof, or a variant of Nicastrin encoded by a nucleic acid that hybridizes to the Nicastrin nucleic acid or its complement under low stringency conditions, wherein said first protein and said second protein are members of a native cellular complex, and wherein said low stringency conditions comprise hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 ug/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS for 1.5 hours at 60° C.
A protein complex comprising Sambiasin-1 (SEQ ID No:
- 1) or Sambiasin-2 (SEQ ID No:
- 4) and Presenilin-1 (SEQ ID No:
- 2) or Presenilin-2 (SEQ ID No:
- 5).
A protein comprising the amino acid sequence of SEQ ID No:
- 1, or a functionally active derivative thereof, or a functionally active fragment thereof, or a homolog thereof, or a variant of Sambiasin-1 encoded by a nucleic acid that hybridizes to the Sambiasin-1 nucleic acid or its complement under low stringency conditions, wherein said low stringency conditions comprise hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 ug/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS for 1.5 hours at 60° C., with the proviso that the protein does not have the amino acid sequence according to SEQ ID No:
- 6.
Protein comprising the amino acid sequence of SEQ ID No:
- 1.
(canceled)
Dependencies are inferred from the claim text.