US 2008/0176800 A1 · METHODS FOR THE DELIVERY OF BIOACTIVE SUBSTANCES WITH BIODEGRADABLE POLYPHOSPHATES

Abstract

The present invention is directed to a biodegradable system for the controlled release of bioactive substances. This system comprises novel biodegradable and biocompatible polyphosphoesters that carry positive charges. Process for making these polyphosphoesters, compositions containing these polyphosphoesters and biologically active substances, articles and methods for delivery of drugs and genes using this system are described. A controlled gene delivery system based on these polyphosphoesters is prepared by complex coacervation of nucleic acid (DNA or RNA) with polymers. The release rates can be manipulated by adjusting the charge ratios of polyphosphoesters to nucleic acids. This gene delivery system yields a higher gene expression in muscle when injected intramuscularly.

Claims17

Dependent claims

A method comprising:

(a) providing a biodegradable polymer composition formed by complexation in aqueous solution comprising:
at least one negatively charged bioactive molecule;
and
a water soluble and positively charged biodegradable polymer that is capable of forming a complex with the at least one negatively charged molecule and comprising the recurring monomeric unit shown in Formula I:
wherein
R1 is a divalent aliphatic organic moiety;
R2 is a positively charged alkyl or heteroalicyclic group selected from the groups consisting of primary amine, secondary amine, tertiary amine, and quaternary amine;
m is an integer from 1 to 6;
n is from 20 to 2,000;
and
(b) administering the composition to an organism under conditions conducive to the delivery of at least a portion of the composition.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/016,186Filed 17 Jan 2008

Priority

14 May 2001

Published

24 Jul 2008

Term ends

Not estimatedThe source records don’t support an estimate.

Classification23 codes

C08L 85/02
Compositions of macromolecular compounds obtained by reactions forming a linkage in the main chain of the macromolecule containing atoms other…:…
A61K 47/605
Obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes: the macromolecule…
A61K 47/645
Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is…:…
A61K 48/0041
Wherein the non-active part clearly interacts with the delivered nucleic acid: the non-active part being polymeric

Family

Application60/290,888Us provisional application · filed 14 May 2001

Application10/499,898Division · filed 14 Feb 2005

Application12/016,186Division

US 2005/0131200 A1Published application

US 7,345,138 B2Related patent

US 2008/0176800 A1This patent

From the US filing history. Foreign family members aren’t listed.

Methods for the delivery of bioactive substances with biodegradable polyphosphates

Life of this patent

Methods for the delivery of bioactive substances with biodegradable polyphosphates

Life of this patent

Abstract

Claims17