Production of Peracids Using An Enzyme Having Perhydrolysis Activity

A process for producing a peroxycarboxylic acid comprising;

• a) providing a set of reaction components, said components comprising:
• 1) a substrate selected from the group consisting of:
• i) esters having the structure
• wherein R1═C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2=C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH2CH2—O)nH or (CH2CH(CH3)—O)nH
• and n=1 to 10;
• and
• ii) glycerides having the structure
• wherein R1═C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
• and
• iii) acetylated saccharides selected from the group consisting of acetylated monosaccharides, acetylated disaccharides, and acetylated polysaccharides;
• 2) a source of peroxygen;
• and
• 3) an enzyme catalyst having perhydrolysis activity, wherein said enzyme catalyst comprises an enzyme comprising a signature motif that aligns with a reference sequence SEQ ID NO:
• 2 using CLUSTALW, said signature motif comprising:
• i) an RGQ motif at amino acid positions 118-120 of SEQ ID NO:2;
• ii) a GXSQG motif at amino acid positions 179-183 of SEQ ID NO:2;
• and
• iii) an HE motif at amino acid positions 298-299 of SEQ ID NO:2;
• and
• b) combining said reaction components under suitable reaction conditions whereby a peroxycarboxylic acid is produced.

A process to disinfect a hard surface or inanimate object using an enzymatically-produced peroxycarboxylic acid composition, said process comprising:

• a) providing a set of reaction components, said components comprising:
• 1) a substrate selected from the group consisting of:
• i) esters having the structure
• wherein R1═C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R2=C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH2CH2—O)nH or (CH2CH(CH3)—O)nH
• and n=1 to 10;
• and
• ii) glycerides having the structure
• wherein R1=C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
• iii) acetylated saccharides selected from the group consisting of acetylated monosaccharides, acetylated disaccharides, and acetylated polysaccharides;
• 2) a source of peroxygen;
• and
• 3) an enzyme catalyst having perhydrolysis activity, wherein said enzyme catalyst comprises an enzyme comprising a signature motif that aligns with a reference sequence SEQ ID NO:
• 2 using CLUSTALW, said signature motif comprising:
• i) an RGQ motif at amino acid positions 118-120 of SEQ ID NO:2;
• ii) a GXSQG motif at amino acid positions 179-183 of SEQ ID NO:2;
• and
• iii) an HE motif at amino acid positions 298-299 of SEQ ID NO:2;
• b) combining said reaction components under suitable aqueous reaction conditions whereby a peroxycarboxylic acid product is formed;
• c) optionally diluting said peroxycarboxylic acid product;
• and
• d) contacting said hard surface with the peroxycarboxylic acid produced in step b) or step c) whereby said hard surface or said inanimate object is disinfected.

A disinfection formulation comprising:

• a) a first mixture comprising an enzyme catalyst comprising a perhydrolase enzyme having a CE-7 signature motif and a substrate selected from the group consisting of methyl lactate, ethyl lactate, methyl glycolate, ethyl glycolate, methyl methoxyacetate, ethyl methoxyacetate, methyl 3-hydroxybutyrate, ethyl 3-hydroxybutyrate, monoacetin, diacetin, triacetin, monopropionin, dipropionin, tripropionin, monobutyrin, dibutyrin, tributyrin, glucose pentaacetate, xylose tetraacetate, acetylated xylan, β-D-ribofuranose-1,2,3,5-tetraacetate, tri-O-acetyl-D-galactal, and tri-O-acetyl-glucal, acetylated monosaccharides, acetylated disaccharides, acetylated polysaccharides, and mixtures thereof;
• said first mixture optionally comprising a further component selected from the group consisting of an inorganic or organic buffer, a corrosion inhibitor, a wetting agent, and combinations thereof;
• and
• b) a second mixture comprising a source of peroxygen and water, said second mixture optionally further comprising a chelating agent.