Method of inducing differentiation of mesenchymal stem cells into neurons
AssigneeSeoul National University Industry Foundation
InventorsMi-Sook Chang, Jung Sun Cho
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Abstract
The present invention relates to a method for inducing differentiation of bone marrow-derived mesenchymal stem cells into mature neurons by culturing them in an optimal medium supplemented with necessary composition. According to the pre-induction method of the invention and a method for inducing differentiation of mesenchymal stem cells into neurons by culturing them in neuronal induction media (NIM) containing butyl hydroxyanisole, forskolin and VPA, mesenchymal stem cells can be effectively differentiated into neurons or motor neurons, which thereby can be effectively used as a therapeutic agent for cell therapy for neurodegenerative diseases.
Claims8
A method for inducing differentiation of mesenchymal stem cells into neurons, comprising the following steps:
- 1) Performing pre-induction of mesenchymal stem cells twice;
- and
- 2) Inducing differentiation of the pre-differentiated mesenchymal stem cells of step 1) in a neuronal induction medium containing butylated hydroxyansiole (BHA), forskolin and valproic acid (VPA) for 2˜8 hours.
A neuronal induction medium containing butylated hydroxyanisole (BHA), forskolin and valproic acid (VPA) to induce differentiation of pre-induced mesenchymal stem cells into neurons.
Dependencies are inferred from the claim text.