In vitro maturation of immature human oocytes
AssigneeMcGill University
InventorRi-Cheng Chian
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Abstract
The invention provides methods for in vitro maturation of immature human oocytes. In one aspect, the invention provides a method for in vitro maturation of immature human oocytes, comprising the steps of: (a) inducing in a female human subject an increase in endogenous luteinizing hormone levels, said subject having not undergone an ovarian stimulation protocol prior to said inducing step; (b) obtaining from said subject an immature oocyte; and (c) culturing said oocyte until maturity.
Claims30
A method for in vitro maturation of immature human oocytes, comprising culturing an immature human oocyte in a culture medium comprising:
- at least one inorganic salt;
- essential amino acids or a source thereof;
- an energy source;
- and
- at least one growth factor.
A method for in vitro maturation of immature human oocytes, comprising culturing an immature human oocyte in a culture medium comprising the inorganic salts, amino acids, vitamins and other components as set forth in Table 2.
Dependencies are inferred from the claim text.