Selective Lysis of Sperm Cells
AssigneeApplera Corporation
InventorYingjie Liu
Life of this patent
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Abstract
The teaching provides a method for selectively lysing sperm cells in a mixed cell sample, particularly in the field of forensic sciences. Reagents and kits for carrying out the methods are also provided.
Claims24
A method of selectively lysing a sperm cell in a mixture of at least one type of non-sperm cell comprises:
- i) incubating the mixture with the selective sperm lysis buffer, wherein the buffer comprises at least on disulfide bond reducing reagent and at least one salt reagent, wherein the salt reagent is at a concentration to lyse a sperm cell whereas a non-sperm cell is intact.
- ii) lysing the sperm cell, while the non-sperm cell is intact, and
- iii) separating the non-sperm cell from the lysed sperm cell.
A method of differential extraction of sperm cell DNA from a cell mixture from a sample carrier, wherein the cell mixture comprises a sperm cell and at least one type of non-sperm cell, comprises:
- i) washing the cell mixture with washing solution and remove the supernatant,
- ii) incubating the mixture with the selective sperm lysis buffer, wherein the buffer comprises at least one disulfide bond reducing reagent and at least one salt reagent, wherein the salt reagent is at a concentration to lyse a sperm cell whereas a non-sperm cell is intact.
- ii) lysing the sperm cell, while the non-sperm cell is intact, and
- iii) separating the non-sperm cell from the lysed sperm cell.
A kit for isolating sperm DNA from non-sperm cell DNA comprises (i) a selective sperm lysis buffer comprising at least one disulfide bond reducing agent and at least one salt, wherein the salt is at the concentration wherein the sperm cell is lysed while the other cells are intact.
Dependencies are inferred from the claim text.