Mitigation of photodamage in analytical reactions
AssigneePacific Biosciences of California, Inc.
InventorsJohn Eid, Devon Murphy, Geoffrey Otto, Stephen Turner
Life of this patent
Estimating the term…
Abstract
Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.
Claims15
1-33. (canceled)
A system for analyzing an illuminated reaction that is susceptible to photodamage when illuminated for a period longer than an photodamage threshold period, comprising:
- a substrate having reagents for the reaction disposed thereon, the reagents comprising a first reactant, a second reactant and a photodamage mitigating agent, wherein the photodamage mitigating agent reduces an amount of photodamage to the first reactant resulting from interaction of the first reactant with the second reactant under excitation illumination that would occur in the absence of the photodamage mitigating agent;
- a mounting stage supporting the substrate and configured to receive the substrate;
- an optical train positioned to be in optical communication with at least a portion of the substrate to illuminate the portion of the substrate and detect signals emanating therefrom;
- a translation system operably coupled to the mounting stage or the optical train for moving one of the optical train and the substrate relative to the other.
35-38. (canceled)
A system for analyzing an illuminated reaction that is susceptible to photodamage when illuminated for a period longer than an photodamage threshold period, comprising:
- a substrate having reagents for the reaction disposed thereon;
- a mounting stage supporting the substrate and configured to receive the substrate;
- an optical train positioned to be in optical communication with at least a first portion of the substrate to illuminate the first portion of the substrate and detect signals emanating therefrom;
- a translation system operably coupled to the mounting stage or the optical train for moving one of the optical train and the substrate relative to the other, wherein the translation system is programmed to move one of the optical train or substrate relative to the other, to illuminate a second portion of the substrate, such that the first portion of the substrate is illuminated for less than the photodamage threshold period.
Dependencies are inferred from the claim text.