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US 2008/0176312 A1Published application
Composition Exhibiting a von Willebrand Factor (vWF) Protease Activity Comprising a Polypeptide Chain with the Amino Acid Sequence AAGGILHLELLV
AssigneeBaxter Aktiengesellschaft
InventorsBernhard Laemmle, Helena Elisabeth Gerritsen, Miha Furlan, Peter Turecek, Hans-Peter Schwarz, Friedrich Scheiflinger, Gerhard Antoine, Randolf Kerschbaumer, Luigina Tagliavacca, Klaus Zimmermann
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Abstract
The invention relates to vWF cleaving entities having a molecular weight of 180 kD, 170 kD, 160 kD, 120 kD or 110 kD and an N-terminal amino acid sequence of AAGGILHLELLV, vWF cleaving complexes and methods for their production.
Claims9
A method for purification of a vWF protease polypeptide comprising the steps of:
- (a) contacting a solution comprising vWF protease polypeptide with an antibody that:
- binds to a polypeptide present in human plasma that exhibits vWF protease activity, has a molecular weight from about 180 kD to about 120 kD as determined by SDS-PAGE under reducing conditions, and comprises the amino acid sequence AAGGILHLELLV (SEQ ID NO:
- 1);
- wherein the polypeptide is contacted with the antibody under physiological conditions whereby the polypeptide binds to the antibody,
- (b) eluting the vWF protease polypeptide from the antibody.
2 more elements
Dependencies are inferred from the claim text.