Method of isolating cell or sample to be analyzed in cell
AssigneeOlympus Optical Co., Ltd.
InventorsKazuo Washiyama, Shinichi Dosaka
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Abstract
There is disclosed a method of irradiating a specimen which is laid on a substrate and which includes a cell with a laser beam to remove an unnecessary specimen portion other than a cell portion from the substrate, next inserting the cell portion into one opening in a cylindrical member to dispose the cylindrical member onto the substrate, next injecting a stripping agent into a container formed by the substrate and cylindrical member to strip the cell portion from the substrate, and sampling the cell portion which floats in the stripping agent.
Claims25
A cell isolating method comprising:
- irradiating a specimen which is laid on a substrate and which includes cells with an ultraviolet light to change properties of an unnecessary specimen portion other than a cell portion;
- using a cylindrical member including openings in opposite ends, and inserting the cell portion into one opening in the cylindrical member to dispose the cylindrical member onto the substrate;
- injecting a stripping agent into a container formed by the substrate and cylindrical member to strip the cell portion from the substrate;
- and
- sampling the cell portion which floats in the stripping agent.
A method of isolating cells or samples to be analyzed in the cells, comprising:
- irradiating a specimen which is laid on a substrate and which includes cells with an ultraviolet light to change properties of an unnecessary specimen portion other than a cell portion;
- using a cylindrical member including openings in opposite ends, and inserting the cell portion into one opening in the cylindrical member to dispose the cylindrical member onto the substrate;
- injecting a solution for eluting the sample existing in the cells into a container formed by the substrate and cylindrical member;
- and
- sampling the sample eluted in the solution.
Dependencies are inferred from the claim text.