Process for the biological production of 1,3-propanediol with high yield
AssigneeE.I. duPont de Nemours and Company
InventorsMARGUERITE A. CERVIN, Philippe Soucaille, Fernando Valle
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Abstract
The present invention provides a microorganism useful for biologically producing 1,3-propanediol from a fermentable carbon source at higher yield than was previously known. The complexity of the cofactor requirements necessitates the use of a whole cell catalyst for an industrial process that utilizes this reaction sequence to produce 1,3-propanediol. The invention provides a microorganism with disruptions in specified genes and alterations in the expression levels of specified genes that is useful in a higher yielding process to produce 1,3-propanediol.
Claims4
A method for the bioproduction of 1,3-propanediol comprising contacting with a suitable carbon substrate under suitable conditions a E. coli strain comprising:
- a) a disrupted endogenous phosphoenolpyruvate-glucose phosphotransferase system comprising one or more of:
- i) a genetically disrupted endogenous ptsH gene preventing expression of active phosphocarrier protein;
- ii) a genetically disrupted endogenous ptsl gene preventing expression of active phosphoenolpyruvate-protein phosphotransferase;
- and
- iii) a genetically disrupted endogenous crr gene preventing expression of active glucose-specific IIA component;
- b) a genetically up regulated endogenous galP gene encoding active galactose-proton symporter, said up regulation resulting in an increased galactose-proton symporter activity;
- c) a genetically up regulated endogenous glk gene encoding active glucokinase, said up regulation resulting in an increased glucokinase activity;
- and
- d) a genetically down regulated endogenous gapA gene encoding active glyceraldehyde 3-phosphate dehydrogenase, said down regulation resulting in a reduced glyceraldehyde 3-phosphate dehydrogenase activity;
- whereby said E. coli strain is capable of bioconverting a suitable carbon source to 1,3-propanediol.
Dependencies are inferred from the claim text.