PEF-TS Expression Units
AssigneeBASF SE
InventorsOskar Zelder, Corinna Klopprogge, Burkhard Kroger, Hartwig Schroder, Stefan Haefner
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Abstract
The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
Claims54
A method of regulating the transcription of a gene comprising introducing into a host cell the nucleic acid molecule of claim 5 or a nucleic acid molecule consisting of SEQ ID NO:1, wherein the nucleic acid molecule has promoter activity.
A method of regulating the expression of a gene comprising introducing into a host cell the expression unit of claim 6 or an expression unit consisting of SEQ ID NO:2.
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An isolated nucleic acid molecule having promoter activity, selected from the group consisting of
- A) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- B) a nucleic acid molecule comprising a nucleotide sequence of at least 90% identity to the entire nucleotide sequence of SEQ ID NO:1;
- C) a nucleic acid molecule which hybridizes with the complement of the nucleotide sequence of SEQ ID NO:1;
- and
- D) a nucleic acid molecule comprising a fragment of the nucleic acid molecule of (A), (B) or (C);
- wherein the nucleic acid molecule does not consist of SEQ ID NO:1.
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An expression unit which enables genes to be expressed in bacteria of the genus Corynebacterium or Brevibacterium, comprising the nucleic acid sequence of SEQ ID NO:21.
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Dependencies are inferred from the claim text.