Two helix binders
AssigneeGeneral Electric Company
InventorsFaisal Ahmed Syud, Jack Mathew Webster
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Abstract
Provided herein are isolated polypeptides derived from the staphylococcal protein A protein B domain comprising a pair of anti-parallel alpha helices that are capable of binding a target. Also provided are nucleic acid sequences encoding such two helix binders, vectors containing the nucleic acid sequences encoding for two helix binders, and host cells transformed with vectors containing the nucleic acid sequences encoding for the two-helix binders. Also provided are methods of using the two helix binders.
Claims32
An isolated polypeptide comprising a two helix segment derived from the Z domain of protein A stabilized with a disulfide bond between two members of a thiol-containing pair of residues, wherein at least one member of the disulfide pair is positioned outside of the binding interface of the polypeptide.
A method of binding a target in a sample comprising applying an isolated polypeptide comprising a two helix segment derived from the Z domain of protein A stabilized with a disulfide bond between two members of a thiol-containing pair of residues, wherein at least one member of the disulfide pair is positioned outside of the binding interface of the polypeptide to a sample containing the target.
Dependencies are inferred from the claim text.