Loading patent…
OwnerJames L. Mohler, Mark Titus +1inventors
InventorsJames L. Mohler, Mark Titus, Kenneth Tomer
Estimating the term…
Provided is a method for determining the presence or amount of 5-α-dihydro-3-keto steroids (ADKSs) comprising the steps of preparing a sample for chromatographic separation, subjecting the sample to chromatographic separation, subjecting the chromatographically separated sample to atmospheric pressure photoionization (APPI), and detecting the amount of alkylated parent ions using mass spectrometry or methylated daughter ions by mass spectrometry after collisionally induced dissociation of the alkylated parent ions. Tandem mass spectrometry of ADKSs with a 6-member A ring and a 3-keto group using APPI and methanol as the alkoxy donor will produce methylated daughter ions with a mass/charge ratio of 85.0. Quantitation of this alkylated daughter ion provides a method to detect very low levels of ADKSs such as DHT. This method can also be used for the simultaneous detection of ADKSs and non-ADKSs.
A method of simultaneous detection of steroids in a test sample said steroids comprising non-5-α-dihydro-3-keto steroids (non-ADKSs) and 5-α-dihydro-3-keto steroids (ADKSs) comprising the steps of:
A method for determining the amount of 5-α-dihydro-3-keto steroids (ADKSs) in a sample comprising the steps of:
Dependencies are inferred from the claim text.