US 2008/0176267 A1 · Method For Obtaining Primary Culture Tumour Cells From Tumour Tissue, In Particular Breast Carcinoma, Primary-Culture Tumour Cells And Their Use

Abstract

The present invention relates to methods for obtaining primary-culture tumour cells from tumour tissue. More precisely, the present invention relates to a method for obtaining primary-culture tumour cells from tumour tissue, in particular breast carcinoma, where, in one method step, the tumour tissue is divided into tumour-tissue sections of a certain size, and these tissue sections are then cultured under defined conditions. The invention also concerns the primary-culture tumour cells that can be obtained by this method from the tumour tissue in particular primary-culture tumour cells from breast carcinoma Finally, the invention relates to the use of these primary-culture tumour cells for, among other things, determining an individual course of tumour treatment, or for testing and screening of new therapeutic agents against tumours.

Claims27

Dependent claims

A method for obtaining essentially primary culture tumor cells from tumor tissue comprising the steps

a) subdivision of the tumor tissue into tumor tissue pieces of a size with a diameter of about 0.5 mm to about 3 mm and
b) culturing the tumor tissue pieces in medium suitable for tumor cells, characterized in that up to the culturing of the tissue pieces the tumor tissue or the tumor tissue pieces is/are subjected to no treatment with proteases.

Dependencies are inferred from the claim text.

Facts

Application

11/910,541Filed 5 Apr 2006

Priority

DE 10 2005 015 953.27 Apr 2005

Published

24 Jul 2008

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12N 5/0693
Vertebrate cells: tumour cells; Cancer cells
G01N 33/5008
Involving human or animal cells: for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
G01N 33/5011
For testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics: for testing antineoplastic activity
G01N 33/502
For testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics: for testing non-proliferative effects

Method For Obtaining Primary Culture Tumour Cells From Tumour Tissue, In Particular Breast Carcinoma, Primary-Culture Tumour Cells And Their Use

Life of this patent

Method For Obtaining Primary Culture Tumour Cells From Tumour Tissue, In Particular Breast Carcinoma, Primary-Culture Tumour Cells And Their Use

Life of this patent

Abstract

Claims27