Assay for Antibodies
AssigneeGenentech, Inc.
InventorsAnan Chuntharapai, Kyu Hong, Yu-Ju Meng
Life of this patent
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Abstract
The presence and quantity of an antibody of interest in a patient's bloodstream or other biological sample can serve as an important clinical or other analytical or diagnostic tool. ELISA methods, and kits for such assays, as well as anti-idiotypic antibodies and hybridomas producing them, are developed to detect levels of the antibody in biological samples, which are from, for example, animal models and human patients.
Claims47
An enzyme-linked immunosorbent assay (ELISA) method for specifically detecting in a biological sample an antibody of interest that binds to a cell-surface, multi-transmembrane protein comprising an intervening extracellular domain of less than about 75 amino acids, comprising (a) contacting and incubating the biological sample with a capture reagent, wherein the capture reagent is an anti-idiotypic antibody binding to the idiotype of the antibody of interest but not to the idiotype of at least one other antibody in the sample that binds to the protein, so as to bind any of the antibody of interest present in the sample, and (b) contacting the sample, and hence any bound antibody of interest, with a detectable antibody that binds to the antibody of interest, and measuring the level of any of the antibody of interest bound to the capture reagent using a detection means for the detectable antibody.
An antibody 8A3 comprising SEQ ID NOS:7 and 9 for the heavy and light chains, respectively, and obtainable from hybridoma 8A3.10 deposited under ATCC number PTA-5914.
An antibody 8C5 obtainable from hybridoma 8C5.1 deposited under ATCC number PTA-5915.
A hybridoma 8C5.1 or 8A3.10 deposited under ATCC deposit number PTA-5915 or PTA-5914, respectively.
An immunoassay kit for specifically detecting in a biological sample an antibody of interest that binds to a cell-surface, multi-transmembrane protein comprising an intervening extracellular domain of less than about 75 amino acids, the kit comprising:
- (a) a container containing, as a capture reagent, an anti-idiotypic antibody binding to the idiotype of the antibody of interest but not to the idiotype of at least one other antibody in the sample that binds to the protein;
- (b) a container containing a detectable anti-idiotypic antibody that binds to the idiotype of the antibody of interest but not to the idiotype of at least one other antibody in the sample that binds to the protein;
- and (c) instructions for detecting said antibody of interest.
Dependencies are inferred from the claim text.