Mold detection composition and methods
OwnerEdward Sobekinventor
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Abstract
This invention relates to compositions and methods for the detection of mold. Specifically, provided herein are compositions and methods for the detection of mold by detecting differences in light intensity of fluoresence resulting from interaction between the mold and the compositions described herein.
Claims32
A fluorescent staining composition for mold detection, comprising:
- 2,2′-([1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (BBD).
A method for producing a high-viscosity fluorescent staining composition for mold detection, comprising:
- a. mixing 2,2′-([1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (BBD), a gelling agent, a solvent, and a diluent together
- b. solubilizing the mixture in the diluent;
- and
- c. cooling the staining solution below the gelling temperature of the gelling agent.
A method for producing a low-viscosity fluorescent staining composition for a mold detection, comprising:
- a. mixing 2,2′-([1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (BBD), a solvent, and a diluent togther;
- and
- b. solubilizing the mixture in the diluent.
A method of detecting a mold in solution comprising:
- a. contacting the solution with a dye comprising 2,2′-([1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (BBD), a solvent, and a diluent;
- b. exposing the contacted solution to electromagnetic radiation;
- and
- c. analyzing the difference in fluorescence intensity between the contacted and uncontacted solution, whereby an increase in light intensity of fluorescence of above about 45% indicates the presence of mold.
A method of detecting a mold on a surface comprising the steps of:
- a. collecting a sample;
- b. contacting the sample with a dye comprising 2,2′-([1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (BBD), a gelling agent, a solvent, and a diluent;
- c. exposing the contacted sample to electromagnetic radiation;
- and
- d. analyzing the difference in fluorescence intensity between the collected sample and the contacted sample, whereby an increase in the light intensity of fluorescence of above about 45% indicates the presence of mold.
A method of quantifying mold concentration in a sample, comprising:
- a. contacting the sample containing the mold with 2,2′-([1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (BBD);
- and
- b. comparing the observed light intensity of fluorescence to a standard.
A kit for detecting, quantifying or both of mold, comprising:
- 2,2′-([1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (BBD), packaging material and instructions for detecting, quantifying or both of mold.
Dependencies are inferred from the claim text.