Phage display affinity and specificity filters and forward screen
AssigneeAmbit Biosciences Corporation
InventorsDavid J. Lockhart, Daniel Kelly Treiber, Patrick Parvis Zarrinkar
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Abstract
The invention provides an affinity filter for the binding of phage-displayed proteins to dissolved target molecules. The phage-displayed proteins are contacted with immobilized target in the presence and absence of dissolved target; the behavior of the phage-displayed proteins as a function of concentration of dissolved target permits approximation of the affinity of the phage-displayed protein for target. The invention also provides a method to screen large numbers of compounds for their ability to compete with a compound known to bind a phage-displayed protein.
Claims5
A method to identify a protein that binds to a first target compound but fails to bind to at least a second compound which method comprises
- contacting a multiplicity of phage-displayed proteins with a solid support to which said first target compound has been immobilized in the presence of a sufficient concentration of said second compound to substantially prevent phage displaying proteins which bind both said first target compound and second compound from being retained on said solid support;
- and
- recovering phage displaying protein from said solid support;
- whereby said recovered phage-displayed protein is identified as binding specifically said first target compound, but not said second compound.
Dependencies are inferred from the claim text.