Apoptin-associating proteiins
AssigneeLeadd B.V.
InventorsMathieu Hubertus Maria Noterorn, Astrid Adriana Anna Maria Danen-Van Oorschot
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Abstract
The invention relates to the field of apoptosis. The invention provides novel therapeutic possibilities, for example novel combinatorial therapies or novel therapeutic compounds that can work alone, sequentially to, or jointly with apoptin, especially in those cases wherein p53 is (partly) non-functional.
Claims23
-11. (canceled)
An isolated or recombinant apoptin-associating proteinaceous substance that induces apoptosis and comprises amino acids 54 to 248 of SEQ ID NO:6.
(canceled)
-26. (canceled)
A method of detecting the presence of cancer cells or cells that are cancer prone in a sample of cells, said method comprising:
- transfecting cells in said sample with a nucleic acid sequence encoding an apoptin-associating proteinaceous substance, wherein said apoptin-associating proteinaceous substance comprises amino acid positions 23 to 250 of SEQ ID NO:
- 6 or a functional fragment thereof, which functional fragment is able to induce apoptosis and which comprises amino acids 54 to 248 of SEQ ID NO:
- 6;
- and
- determining the percentage of apoptosis of cells in said sample, wherein a decrease in apoptosis as compared to normal cells is indicative of the presence of cancer cells or cells that are cancer prone.
A method of detecting the presence of cancer cells or cells that are cancer prone in a sample of cells, said method comprising:
- transfecting said cells in said sample with a nucleic acid sequence encoding an apoptin-associating proteinaceous substance, wherein said apoptin-associating proteinaceous substance comprises amino acid positions 23 to 250 of SEQ ID NO:
- 6 or a functional fragment thereof, which functional fragment is able to induce apoptosis and which comprises amino acids 54 to 248 of SEQ ID NO:
- 6;
- and
- determining the intracellular localization of a proteinaceous substance derived from said isolated or recombinant nucleic acid sequence in cells in said sample, wherein localization of said proteinaceous substance in the nucleus is indicative of the presence of cancer cells or cells that are cancer prone.
A method of identifying a putative cancer-inducing agent, said method comprising:
- exposing a sample of cells to a cancer-inducing agent;
- contacting said sample of cells with a nucleic acid sequence encoding an apoptin-associating proteinaceous substance, wherein said apoptin-associating proteinaceous substance comprises amino acid positions 23 to 250 of SEQ ID NO:
- 6 or a functional fragment thereof, which functional fragment is able to induce apoptosis and which comprises amino acids 54 to 248 of SEQ ID NO:
- 6;
- and
- detecting the presence of cancer cells or cells that are cancer prone by determining a percentage of apoptosis of cells in said sample of cells, wherein said percentage is indicative of the carcinogenesis of said agent.
(canceled)
A process for producing a peptide comprising the peptide of SEQ ID NO:6, the process comprising:
- recombinantly expressing an isolated nucleic acid sequence encoding the peptide of SEQ ID NO:6 in a cell.
(canceled)
Dependencies are inferred from the claim text.