Nucleic acid enzyme biosensors for ions
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Jing Li
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Abstract
Disclosed are compositions and methods for the sensitive and selective detection of ions using nucleic acid enzymes.
Claims22
A method of detecting the presence of an ion comprising:
- (a) contacting a nucleic acid enzyme, the enzyme dependent on the ion to produce a product from a substrate, with a sample suspected of containing an ion;
- and
- (b) measuring the product of the nucleic acid enzymatic reaction.
13-41. (canceled)
A method of determining the concentration of an ion in a sample comprising:
- (a) contacting a nucleic acid enzyme, the enzyme dependent on the ion to produce a product from a substrate, with a sample containing an unknown concentration of an ion;
- (b) measuring the product of the nucleic acid enzymatic reaction;
- and
- (c) comparing the measurement obtained (b) with that of a standard curve created using known concentrations of the ion.
A biosensor comprising:
- (a) a nucleic acid enzyme dependent on an ion to produce a product;
- (b) a quencher;
- and
- (c) a photodetector.
44-46. (canceled)
A biosensor comprising:
- (a) a nucleic acid enzyme dependent on an ion to produce a product,
- (b) a fluorophore;
- and
- (c) a photodetector.
A composition comprising a nucleic acid enzyme linked to a fluorophore.
A composition comprising a nucleic acid enzyme linked to a quencher.
A composition comprising a nucleic acid enzyme, substrate, fluorophore, and quencher.
A composition comprising a substrate for a nucleic acid enzyme linked to a quencher.
A composition comprising a substrate for a nucleic acid enzyme linked to a fluorophore.
Dependencies are inferred from the claim text.