Membrane bound reporter gene system
OwnerSteve ROFFLER, Tian-Lu Cheng +1inventors
InventorsSteve ROFFLER, Tian-Lu Cheng, Yu-Cheng Su
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Abstract
A recombinant DNA construct is provided and includes a first DNA fragment encoding a β-glucuronidase and a second DNA fragment encoding a membrane anchoring domain. The β-glucuronidase may be a human β-glucuronidase or a mouse β-glucuronidase. In one embodiment, an expression vector for delivering a gene of interest or portion thereof into a host cell includes a DNA sequence for the gene of interest, a first DNA fragment encoding a β-glucuronidase, and a second DNA fragment encoding a membrane anchoring domain. In another embodiment, a method of introducing a gene of interest or portion thereof into a host cell is provided, including introducing into the host cell a recombinant DNA construct.
Claims32
A recombinant DNA molecule comprising a first DNA fragment encoding a β-glucuronidase and a second DNA fragment encoding a membrane anchoring domain.
A method of introducing a gene of interest or portion thereof into a host cell, comprising:
- introducing into the host cell a recombinant DNA construct, the recombinant DNA construct comprising
- a first DNA fragment encoding a β-glucuronidase;
- and
- a second DNA fragment encoding a membrane anchoring domain.
An expression vector for delivering a gene of interest or portion thereof into a host cell, comprising:
- a first DNA fragment encoding a β-glucuronidase;
- and
- a second DNA fragment encoding a membrane anchoring domain.
A method of imaging the expression of a gene of interest in a host cell, comprising:
- introducing into the host cell a recombinant DNA construct, the recombinant DNA construct comprising
- a DNA sequence for the gene of interest or portions thereof;
- a first DNA fragment encoding a β-glucuronidase;
- and
- a second DNA fragment encoding a membrane anchoring domain;
- providing a β-glucuronidase substrate capable of being converted into a report reporter product by the β-glucuronidase;
- and
- monitoring the levels of the reporter product in the host cell.
Dependencies are inferred from the claim text.