Back to results
US 2008/0176222 A9Published application
Multiplex PCR for the detection of AmpC beta-lactamase genes
AssigneeCreighton University
InventorsNancy Hanson, Francisco-Javier Perez Perez
Life of this patent
Estimating the term…
Abstract
Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain AmpC beta-lactamases. The primers can be employed in methods to detect the presence or absence of an AmpC beta-lactamase gene in samples, and to identify nucleic acid characteristic of AmpC beta-lactamase genes in samples, particularly, in clinical isolates of Gram-negative bacteria.
Claims20
A primer selected from the group consisting of:
- 5′-GCTGCTCAAGGAGCACAGGAT-3′;
- (SEQ ID NO:1)
- 5′-CACATTGACATAGGTGTGGTGC-3′;
- (SEQ ID NO:2)
- 5′-TGGCCAGAACTGACAGGCAAA-3′;
- (SEQ ID NO:3)
- 5′-TTTCTCCTGAACGTGGCTGGC-3′;
- (SEQ ID NO:4)
- 5′-AACTTTCACAGGTGTGCTGGGT-3′;
- (SEQ ID NO:5)
- 5′-CCGTACGCATACTGGCTTTGC-3′;
- (SEQ ID NO:6)
- 5′-AACAGCCTCAGCAGCCGGTTA-3′;
- (SEQ ID NO:7)
- 5′-TTCGCCGCAATCATCCCTAGC-3′;
- (SEQ ID NO:8)
- 5′-TCGGTAAAGCCGATGTTGCGG-3′;
- (SEQ ID NO:9)
- 5′-CTTCCACTGCGGCTGCCAGTT-3′;
- (SEQ ID NO:10)
- 5′-AACATGGGGTATCAGGGAGATG-3′;
- (SEQ ID NO:11)
- 5′-CAAAGCGCGTAACCGGATTGG-3′;
- (SEQ ID NO:12)
- and complements thereof.
22 more elements
2-25. (canceled)
A diagnostic kit for identifying ampC beta-lactamase nucleic acid, wherein the kit comprises:
- a) at least two primer pairs capable of hybridizing to a specific type of ampC beta-lactamase nucleic acid;
- b) at least one positive control and at least one negative control;
- and
- c) a protocol for identification of an ampC beta-lactamase nucleic acid.
1 more element
Dependencies are inferred from the claim text.