Polypeptide films and methods
AssigneeArtificial Cell Technologies, Inc.
InventorDonald Templeton Haynie
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Abstract
Disclosed herein are polypeptide multilayer films wherein a therapeutic agent is covalently linked to a first layer polypeptide. An advantage of such attachment is that the linked therapeutic agent can be controllably released from the multilayer film into the environment of the film upon the addition of a suitable stimulus. An advantage of the disclosed films and methods is enablement of environmentally-stimulated release under specific conditions.
Claims20
A multilayer film, the film comprising two or more layers of polyelectrolytes, wherein adjacent layers comprise oppositely charged polyelectrolytes,
- wherein a first layer polyelectrolyte comprises a first layer polypeptide comprising one or more first amino acid sequence motifs,
- wherein the one or more first amino acid sequence motifs consists of 5 to
- 15 amino acids and has a net charge per residue of 0.4, wherein the first layer polypeptide is at least 15 amino acids long, and has a balance of charge at pH 7 greater than or equal to approximately one-half of the total length of the first layer polypeptide;
- wherein the first layer polypeptide comprises a therapeutic agent reversibly linked to the first layer polypeptide;
- and
- wherein a second layer comprises a second layer polyelectrolyte comprising a polycationic material or a polyanionic material having a molecular weight of greater than 1,000 and at least 5 charges per molecule, wherein the second layer does not comprise a polypeptide.
The multilayer film of claim 6, wherein the functional pendant group comprises a carboxylate, an alcohol, a thiol, an oxime, a hydraxone, a hydrazide, or an amine group.
A method of assembling a polypeptide multilayer film, comprising
- disposing a second layer polyelectrolyte onto a substrate, wherein the second layer polyelectrolyte comprising a polycationic material or a polyanionic material having a molecular weight of greater than 1,000 and at least 5 charges per molecule, wherein the second layer does not comprise a polypeptide;
- and
- disposing a first layer polypeptide on the second layer polyelectrolyte, wherein the first layer polypeptide comprises one or more first amino acid sequence motifs,
- wherein the one or more first amino acid sequence motifs consists of 5 to
- 15 amino acids and has a net charge per residue of 0.4, wherein the first layer polypeptide is at least 15 amino acids long, and has a balance of charge at pH 7 greater than or equal to approximately one-half of the total length of the first layer polypeptide;
- and
- wherein the first layer polypeptide comprises a therapeutic agent reversibly linked to the first layer polypeptide.
Dependencies are inferred from the claim text.