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US 2008/0175870 A1Published application
Human Cancer Stem Cells
AssigneeMacroGenics West, Inc.
InventorsJennie P. Mather, Penelope Roberts
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Abstract
This invention discloses isolated populations of human cancer stem cells. Methods for characterizing, isolating and culturing human cancer stem cells are also disclosed. Uses for human cancer stem cells are provided.
Claims13
A population of human cancer stem cells that are isolated from human tumor tissue, wherein said cells are grown in a nutrient media optimized for their growth.
A method of isolating a population of human cancer stem cells comprising:
- (a) dissociating a source of human cancer stem cells;
- (b) placing the dissociated source of human cancer stem cells in a nutrient media that has been optimized for the growth of human cancer stem cells;
- (c) maintaining suitable culture conditions sufficient to support the growth of human cancer stem cells from the source of human cancer stem cells into said nutrient media;
- (d) subculturing said population of human cancer stem cells to obtain a substantially pure population of human cancer stem cells.
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