Methods and compositions for increasing replication capacity of an influenza virus
AssigneeMedImmune Vaccines, Inc.
InventorsHong Jin, Bin Liu
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Abstract
In certain aspects, the present invention provides methods for increasing the replication capacity of influenza viruses in hens' eggs and/or cell culture, recombinant and/or reassortant influenza viruses with increased replication capacity, and immunogenic and vaccine compositions comprising such recombinant and/or reassortant influenza viruses. In other aspects, the invention further provides nucleic acids encoding influenza genes associated with increased replication capacity, expression vectors comprising the nucleic acids of the invention, methods for making influenza viruses with increased replication capacity, and kits useful for practice of the methods.
Claims29
A method for increasing the replication capacity of an influenza virus, comprising altering an at least one amino acid of the influenza virus M1 protein, wherein the at least one amino acid is at a position corresponding to at least one position 95, 143, 144 or 218 of the M1 protein of influenza strain MDV-A as shown in SEQ ID NO:1;
- wherein the altering at the position corresponding to position 95 is to a lysine;
- wherein the altering at the position corresponding to position 143 is to an alanine;
- wherein the altering at the position corresponding to position 144 is to a phenylalanine;
- or
- wherein the altering at the position corresponding to position 218 is to an isoleucine;
- and
- wherein the altering increases thereby increasing the replication capacity of the influenza virus.
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The method of claim 12 wherein the altering is of amino acids at positions corresponding to positions 95, 143, 144 and 218.
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A reassortant influenza virus comprising an M1 protein, wherein the M1 protein comprises an alteration in at least one amino acid at a position corresponding to position 95, 143, 144, or 218 of the M1 protein of influenza strain MDV-A as shown in SEQ ID NO:
- 1
- wherein the alteration of the position corresponding to position 95 is to lysine,
- wherein alteration at the position corresponding to position 143 is to alanine,
- wherein alteration at the position corresponding to position 144 is to phenylalanine, and
- wherein alteration at the position corresponding to position 218 is to isoleucine.
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Dependencies are inferred from the claim text.