Immunoreactive peptides from epstein-barr virus
AssigneesOrtho Diagnostics Systems, Inc., Georgetown University
InventorsRichard S. Smith, Gary R. Pearson, D. Elliot Parks, Susan Pothen Varghese
Life of this patent
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Abstract
Epstein-Barr virus (EBV) specific polypeptides are disclosed. Also disclosed are the use of these polypeptides for the production of polypeptide-specific antibodies and the diagnosis and treatment of EBV-associated disease.
Claims27
A monoclonal antibody to a polypeptide consisting of an amino acid sequence having the formula
- (φ)n
- wherein n is 1 to about 1000 and φ is 25 amino acids or less and has the formula:
- (α ETFTETWNRFITHTE β)n
- (SEQ. ID NO:
- 1)
- (α GMLEASEGLDGWIHQ β)n
- (SEQ. ID NO:
- 2)
- (α HQQGGWSTLIEDNIP β)n
- (SEQ. ID NO:
- 3)
- (α KQKHPKKVKQAFNPL β)n
- (SEQ. ID NO:
- 4)
- wherein α and β are independently from 0 to about 5 naturally occurring amino acids wherein the polypeptide is capable of binding antibody in a specimen from an individual with Epstein-Barr virus (EBV)-associated disease.
A method of detecting an amino acid sequence comprising a polypeptide consisting of an amino acid sequence having the formula:
- (φ)n
- wherein n is 1 to about 1000 and φ is 25 amino acids or less and has the formula:
- (α ETFTETWNRFITHTE β)n
- (SEQ. ID NO:
- 1)
- (α GMLEASEGLDGWIHQ β)n
- (SEQ. ID NO:
- 2)
- (α HQQGGWSTLIEDNIP β)n
- (SEQ. ID NO:
- 3)
- (α KQKHPKKVKQAFNPL β)n
- (SEQ. ID NO:
- 4)
- wherein α and β are independently from 0 to about 5 naturally occurring amino acids wherein the polypeptide is capable of binding antibody in a specimen from an individual with Epstein-Barr virus (EBV)-associated disease,
- the method comprising contacting a specimen suspected of containing the amino acid sequence with a monoclonal antibody to said polypeptide.
A polynucleotide encoding a polypeptide consisting of an amino acid sequence having the formula:
- (φ)n
- wherein n is 1 to about 1000 and φ is 25 amino acids or less and has the formula:
- (α ETFTETWNRFITHTE β)n
- (SEQ. ID NO:
- 1)
- (α GMLEASEGLDGWIHQ β)n
- (SEQ. ID NO:
- 2)
- (α HQQGGWSTLIEDNIP β)n
- (SEQ. ID NO:
- 3)
- (α KQKHPKKVKQAFNPL β)n
- (SEQ. ID NO:
- 4)
- wherein α and β are independently from 0 to about 5 naturally occurring amino acids wherein the polypeptide is capable of binding antibody in a specimen from an individual with Epstein-Barr virus (EBV)-associated disease,
- and polynucleotide sequences complementary thereto.
A method of treating Epstein-Barr virus (EBV)-associated disease in an animal comprising administering to the animal a therapeutically effective amount of a monoclonal antibody to a polypeptide consisting of an amino acid sequence having the formula:
- (φ)n
- wherein n is 1 to about 1000 and φ is 25 amino acids or less and has the formula:
- (α ETFTETWNRFITHTE β)n
- (SEQ. ID NO:
- 1)
- (α GMLEASEGLDGWIHQ β)n
- (SEQ. ID NO:
- 2)
- (α HQQGGWSTLIEDNIP β)n
- (SEQ. ID NO:
- 3)
- (α KQKHPKKVKQAFNPL β)n
- (SEQ. ID NO:
- 4)
- wherein α and β are independently from 0 to about 5 naturally occurring amino acids, wherein the polypeptide is capable of binding antibody in a specimen from an individual with Epstein-Barr virus (EBV)-associated disease.
A method of treating Epstein-Barr virus (EBV)-associated disease in an animal, comprising administering to the animal a immunogenically effective amount of a polypeptide consisting of an amino acid sequence having the formula:
- (φ)n
- wherein n is 1 to about 1000 and φ is 25 amino acids or less and has the formula:
- (α ETFTETWNRFITHTE β)n
- (SEQ. ID NO:
- 1)
- (α GMLEASEGLDGWIHQ β)n
- (SEQ. ID NO:
- 2)
- (α HQQGGWSTLIEDNIP β)n
- (SEQ. ID NO:
- 3)
- (α KQKHPKKVKQAFNPL β)n
- (SEQ. ID NO:
- 4)
- wherein α and β are independently from 0 to about 5 naturally occurring amino acids, wherein the polypeptide is capable of binding antibody in a specimen from an individual with Epstein-Barr virus (EBV)-associated disease.
A kit useful for detection of an amino acid sequence comprising a polypeptide consisting of an amino acid sequence having the formula:
- (φ)n
- wherein n is 1 to about 1000 and φ is 25 amino acids or less and has the formula:
- (α ETFTETWNRFITHTE β)n
- (SEQ. ID NO:
- 1)
- (α GMLEASEGLDGWIHQ β)n
- (SEQ. ID NO:
- 2)
- (α HQQGGWSTLIEDNIP β)n
- (SEQ. ID NO:
- 3)
- (α KQKHPKKVKQAFNPL β)n
- (SEQ. ID NO:
- 4)
- wherein α and β are independently from 0 to about 5 naturally occurring amino acids wherein the polypeptide is capable of binding antibody in a specimen from an individual with Epstein-Barr virus (EBV)-associated disease in a specimen suspected of containing such sequence,
- the kit comprising carrier means being compartmentalized to receive in close confinement therein one or more containers comprising a container containing a monoclonal antibody to said polypeptide.
Dependencies are inferred from the claim text.