Culture-expanded T suppressor cells and methods of use thereof
AssigneeThe Rockefeller University
InventorsRalph M. Steinman, Kristin Tarbell, Sayuri Yamazaki
Life of this patent
Estimating the term…
Abstract
This invention relates to culture-expanded T suppressor cells derived from CD25−CD4+ T cells, and their use in modulating immune responses. This invention provides methods of producing culture-expanded T suppressor cells, which are antigen specific, and their use in modulating autoimmune diseases and transplantation rejection.
Claims37
An isolated, culture-expanded CD25+ CD4+ T suppressor cell population, wherein said population is prepared by the process of exposing CD25−CD4+ T cells to dendritic cells, antigen and TGF-β1.
A method for producing an isolated, culture-expanded CD25+CD4+ T suppressor cell population, comprising:
- a. contacting a population of CD25− CD4+ T cells with dendritic cells;
- an antigen selected from the group consisting of an antigenic peptide, an antigenic protein, and a derivative thereof;
- and TGF-β1, for a period of time resulting in antigen-specific CD25+ CD4+ T cell differentiation and expansion;
- and
- b. isolating the expanded CD25+ CD4+ T cells obtained in (a),
- thereby producing an isolated, culture-expanded CD25+CD4+ T suppressor cell population.
A method for delaying onset, reducing incidence, suppressing or treating autoimmunity, an autoimmune disease or an autoimmune disorder in a subject, comprising the steps of:
- a. contacting a population of CD25− CD4+ T cells with dendritic cells;
- an antigen selected from the group consisting of an antigenic peptide, an antigenic protein, and a derivative thereof;
- and TGF-β1, for a period of time resulting in antigen-specific CD25+ CD4+ T cell differentiation and expansion;
- and
- b. administering the expanded CD25+ CD4+ T cells obtained in (a) to a subject,
- wherein said expanded CD25+ CD4+ T cells inhibit, suppress, downmodulate or prevent an autoimmune response in said subject, thereby delaying onset, reducing incidence, suppressing or treating autoimmunity.
A method for delaying onset, reducing incidence, suppressing or treating autoimmunity, an autoimmune disease or autoimmune disorder in a subject, comprising the step of contacting a dendritic cell population in vivo with an antigenic peptide or protein associated with an autoimmune response in said subject, or a derivative thereof, and TGF-β1 for a period of time whereby said dendritic cells contact CD25− CD4+ T cells in said subject, inducing and stimulating antigen-specific induction and expansion into CD25+ CD4+ T cells in said subject, wherein expanded CD25+ CD4+ T cells suppress an autoimmune response in said subject, thereby delaying onset, reducing incidence, suppressing or treating autoimmunity, an autoimmune disease or an autoimmune disorder.
Dependencies are inferred from the claim text.