Methods and systems for extended in vitro culture of neuronal cells
AssigneeUniversity of Washington
InventorsRyo Kubota, Thomas A. Reh, Andrew J. Fischer
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Abstract
A cell culture system related to extended in vitro culture of mature neuronal cells and methods for preparing the cell culture system are provided. In a preferred embodiment the invention provides a cell culture system comprising a mixture of mature neuronal retinal cells and cells isolated from a ciliary body. Methods for identifying bioactive agents that alter neurodegeneration of neuronal retinal cells are also provided.
Claims21
A cell culture system comprising a mixture of mature neuronal cells and cells isolated from a ciliary body.
A retinal cell culture system comprising a mixture of mature retinal neuronal cells and cells isolated from a ciliary body.
A retinal cell culture system comprising a mixture of mature retinal neuronal cells and cells isolated from a ciliary body, wherein the mature retinal neuronal cells are selected from the group consisting of bipolar cells, horizontal cells, amacrine cells, ganglion cells, and photoreceptor cells.
A retinal cell culture system comprising a mixture of (i) mature retinal neuronal cells;
- (ii) cells isolated from a ciliary body;
- and (iii) embryonic retinal cells.
A method for producing a retinal cell culture system comprising co-culturing a mature retinal neuronal cell and a cell isolated from a ciliary body.
A method for enhancing survival of a mature retinal neuronal cell in vitro comprising co-culturing a mature retinal neuronal cell and a cell isolated from a ciliary body.
A method for treating a retinal disease comprising introducing isolated retinal stem cells into retinal tissue of a subject in need thereof.
Dependencies are inferred from the claim text.