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AssigneeAastrom Bioscience, Inc.
InventorsBrian Hampson, Kristin Goltry, Doug Smith, Jonathan A. Rowley, Naia Venturi
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The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use. In such embodiments, the final composition is a substantially purified and concentrated cell mixture suspended in a physiologic solution suitable for immediate use in humans without further washing, volume reduction, or processing. Embodiments are also applicable to harvesting (and/or washing) particles within a liquid or solution within a chamber.
An isolated cell composition for tissue repair comprising a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the viability of said cells is at least 80% and the composition contains:
A method of modulating an immune response, an inflammatory response or angiogenesis in a patient in need thereof, comprising administering a cultured mixed cell composition to said patient wherein the cultured cell composition produces at least one cytokine selected from the group consisting of interleukin-1 receptor antagonist, interleukin-6, interleukin-8, interleukin 10, vascular endothelial growth factor, monocyte chemoattractant protein-1 angiopoeitin 1, angiopoeitin 2 and hepatocyte growth factor.
A method for processing cultured cells comprising;
A method for harvesting cultured cells comprising:
A method for separating micro-particles from a containing liquid or solution provided in a chamber having a predetermined volume and geometry, comprising:
A composition comprising a mixture of cells with at least 1% of the cells co-express CD90 and CD15 cell surface markers.
Dependencies are inferred from the claim text.