Maintenance and Propagation of Mesenchymal Stem Cells
AssigneeBoard of Trustees of the University of Arkansas
InventorsXiao-Dong Chen, Robert L. Jilka
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Abstract
Various embodiments of the present invention include compositions, materials and methods for maintaining and propagating mammalian mesenchymal stem cells in an undifferentiated state in the absence of feeder cells and applications of the same.
Claims56
A cellular composition of proliferating mammalian mesenchymal stem cells maintained in culture in an undifferentiated state comprising mammalian mesenchymal stem cells growing on an extracellular matrix.
A composition for maintaining mammalian mesenchymal stem cells in culture in an undifferentiated state comprising an extracellular matrix.
A method of maintaining mammalian mesenchymal stem cells in culture in an undifferentiated state comprising culturing mammalian mesenchymal stem cells in the presence of an extracellular matrix.
A cell culture apparatus for maintaining mammalian mesenchymal stem cells in an undifferentiated state comprising a substrate and an extracellular matrix.
A method of maintaining mammalian mesenchymal stem cells in an undifferentiated state comprising culturing mammalian mesenchymal stem cells in the presence of an inhibitor of BMP2 activity.
A bone forming composition comprising:
- (i) a cellular composition of mammalian mesenchymal stem cells maintained in culture in an undifferentiated state comprising mammalian mesenchymal stem cells grown on an extracellular matrix, and (ii) a transplantation vehicle.
A method of generating bone in a patient in need thereof comprising:
- (i) obtaining mammalian mesenchymal stem cells;
- (ii) producing a bone forming composition by culturing said mammalian mesenchymal stem cells in vitro on an extracellular matrix and isolating undifferentiated mammalian mesenchymal stem cells from said culture;
- and
- (iii) administration of said bone forming composition to said patient.
The method of claim 54 wherein said transplantation vehicle comprises a hydroxyapatite ceramic powder.
Dependencies are inferred from the claim text.