Assay for anti-metastatic agents
AssigneeMemorial Sloan Kettering Cancer Center
InventorsJoan Massague, Larry Norton
Life of this patent
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Abstract
This invention relates, e.g., to a method for identifying an agent that inhibits a metastatic cell (e.g. that inhibits cancer metastasis), comprising measuring the amount of seeding of a tumor by a detectably labeled, metastatic cell, in a subject, in the presence and absence of a putative agent, wherein the amount of seeding by the metastatic cell is proportional to the metastatic potential of the cell, and wherein a significant amount of inhibition of the seeding by the putative agent indicates that the putative agent is effective to inhibit the metastatic cell (e, g, cancer metastasis). Also described are kits suitable for performing methods of the invention.
Claims23
A method for identifying an agent that inhibits a metastatic cell, comprising,
- measuring the amount of seeding of a tumor by a detectably labeled, metastatic cell, in a subject, in the presence and absence of a putative agent,
- wherein the amount of seeding by the metastatic cell is proportional to the metastatic potential of the cell, and
- wherein a significant amount of inhibition of the seeding by the putative agent indicates that the putative agent is effective to inhibit the metastatic cell.
A method for identifying an agent that inhibits a metastatic cell, comprising,
- measuring the amount of seeding of a colony of tumorigenic and/or metastatic cells by a detectably labeled, metastatic cell, in vitro, in the presence and absence of a putative agent,
- wherein the amount of seeding by the detectably labeled metastatic cell is proportional to the metastatic potential of the cell, and
- wherein a significant amount of inhibition of the seeding by the putative agent indicates that the putative agent is effective to inhibit the metastatic cell.
A kit for identifying an agent that inhibits a metastatic cell, comprising
- a cell from a tumorigenic cell line;
- cells from one or more metastatic cell lines derived from the tumorigenic cell;
- and, optionally,
- containers to hold the cells.
Dependencies are inferred from the claim text.