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US 2008/0168587 A1Published application
Brassica Juncea Lines With High Oleic Acid Profile In Seed Oil
AssigneeViterra, Inc.
InventorsKening Yao, Derek A. Potts, Daryl Males
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AssigneeViterra, Inc.
InventorsKening Yao, Derek A. Potts, Daryl Males
Estimating the term…
In various aspects, the invention provides Brassica juncea plants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than other B. juncea plants. In one embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-313-1/BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a G to A substitution in the ORF at position 281 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Glycine-94 Aspartic acid mutation in the sequence of the predicted BjFAD2-a protein. In another embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-357-3/BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a C to T substitution in the ORF at position 647 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Proline-216 Leucine mutation in the sequence of the predicted BjFAD2-a protein. As a result of these mutations, it can be predicted that the function of the BjFAD2-a proteins are negatively affected in Brassica juncea lines MJ02-313-1 and MJ357-3 as reflected in the increased levels of oleic acid in seed oil in comparison with the wild-type line J96D-4830. Seeds from MJ02-313-1 and MJ02-357-3 plants may for example yield an oil having oleic acid content of greater than 70% by weight.
A Brassica juncea plant whose seeds have an endogenous fatty acid content comprising at least 70.0% oleic acid by weight, and less than 1.0% erucic acid by weight.
A Brassica juncea seed designated MJ02-313-1, representative sample of seed was deposited under ATCC Accession No. PTA-7955.
A Brassica juncea seed designated MJ02-357-3, representative sample of seed was deposited under ATCC Accession No. PTA-7956.
A Brassica juncea plant, plant cell, or a part thereof, containing the MJ02-313-1/BjFAD2-a mutant allele having nucleic acid sequence SEQ ID NO:2, wherein said allele is also present in a representative sample of seed deposited under ATCC Accession No. PTA-7955.
A Brassica juncea plant, plant cell or a part thereof, containing the MJ02-357-3/BjFAD2-a mutant allele having nucleic acid sequence SEQ ID NO:3, wherein said allele is also present in a representative sample of seed deposited under ATCC Accession No. PTA-7956.
A method for identifying Brassica juncea plants, whose seeds have an endogenous fatty acid content comprising at least 70.0% oleic acid by weight, by determining the presence of the mutant MJ02-313-1/BjFAD2-a allele.
A method for identifying Brassica juncea plants, whose seeds have an endogenous fatty acid content comprising at least 70.0% oleic acid by weight, by determining the presence of the mutant MJ02-357-3/BjFAD2-a allele.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having SEQ ID NO:5.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having SEQ ID NO:6.
A Brassica plant comprising the mutant MJ02-313-1/BjFAD2-a allele or a portion of the said allele effective to confer an increased oleic acid content in seed.
A Brassica plant comprising the mutant MJ02-357-3/BjFAD2-a allele or a portion of the said allele effective to confer an increased oleic acid content in seed.
A method for screening Brassica plants for individual mutant plant line comprising seeds having an endogenous fatty acid content comprising at least 70.0% oleic acid by weight by analyzing leaf tissue.
Dependencies are inferred from the claim text.