Brassica Plant Comprising a Mutant Fatty Acid Desaturase

An isolated FAD2 polypeptide encoded by a nucleic acid, the nucleotide sequence of which comprises a nucleotide deletion, said FAD2 polypeptide being non functional.

A Brassica plant with a high level of oleic acid in its seed oil, comprising a mutant FAD2 allele.

Hybrid Brassica seeds, comprising the mutant FAD2 allele, which develop into plants, wherein the solid component of the seeds of said plants contains less than 30 micromoles of any one or any mixture of 3-butenyl glucosinolate, 4-pentenyl glucosinolate, 3-hydroxy-3 butenyl glucosinolate, and 2-hydroxy-4-pentenyl glucosinolate per gram of air-dry, oil-free solid component, and which produces an seed oil containing less than 2% erucic acid of the total fatty acids in the oil.

A method for detecting the presence or absence of the mutant FAD2 allele in the DNA of Brassica tissue or seeds, comprising performing the mutant FAD2 PCR Identification Protocol.

A kit for the detection of the mutant FAD2 allele in Brassica DNA samples, wherein said kit comprises one or more PCR primer pairs, which are able to amplify a DNA marker linked to the mutant FAD2 allele.

A PCR marker primer for Brassica, selected from the group consisting of OSR144 (SEQ ID No. 7) and OSR145 (SEQ ID No. 8).

Use of any one of PCR markers primers for monitoring the introgression of mutant FAD2 allele in Brassica oilseed plants or for PCR analysis of Brassica oilseed plants.