Signal transduction stress-related proteins and methods of use in plants
AssigneeBASF Plant Science GmbH
InventorsOswaldo da Costa e Silva, Hans J. Bohnert, Nocha Van Thielen, Ruoying Chen, Manabu Ishitani
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Abstract
A transgenic plant transformed by a Signal Transduction Stress-Related Protein (STSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated STSRPs, and isolated nucleic acid coding STSRPs, and vectors and host cells containing the latter.
Claims22
A transgenic plant cell transformed with an expression cassette comprising an isolated polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1800 of SEQ ID NO:10;
- and
- b) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 337 of SEQ ID NO:15;
A transgenic plant transformed with an expression cassette comprising an isolated polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1800 of SEQ ID NO:10;
- and
- b) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 337 of SEQ ID NO:15.
A seed which is true breeding for a transgene comprising a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1800 of SEQ ID NO:10;
- and
- b) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 337 of SEQ ID NO:15.
An isolated polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1800 of SEQ ID NO:10;
- and
- b) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 337 of SEQ ID NO:15.
A method of producing a drought-tolerant transgenic plant, the method comprising the steps of:
- a) transforming a plant cell with an expression cassette comprising a polynucleotide selected from the group consisting of:
- i) a polynucleotide having a sequence comprising nucleotides 1 to 1800 of SEQ ID NO:10;
- and
- ii) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 337 of SEQ ID NO:15;
- b) growing the transformed plant cell to generate transgenic plants;
- and
- c) screening the transgenic plants generated in step b) to identify a transgenic plant that expresses the polypeptide and exhibits increased tolerance to drought stress as compared to a wild type variety of the plant.
Dependencies are inferred from the claim text.