Methods of purifying proteins
OwnerHai Paninventor
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Abstract
The present invention relates to methods of purifying and analyzing preparations of Fc domain containing polypeptides comprising binding said polypeptide to protein A, more particularly to a protein A column, and eluting with a pH gradient elution system. This method enhances separation of aggregates, multimers and modified versions of the protein from the single Fc domain containing polypeptide.
Claims13
A method of purifying a human Fc domain containing polypeptide comprising binding said polypeptide to protein A and eluting with a pH gradient.
The product purified by a method comprising purifying an Fc domain containing polypeptide comprising binding said polypeptide to protein A and eluting with a pH gradient.
The product of claim 13, wherein the antibody does not have oxidized methionines in the Fc domain.
Dependencies are inferred from the claim text.