Methods for Modulating the Efficacy of Nucleic Acid Based Therapies
OwnerDayton T. Reardan, Larry J. Smith +1inventors
InventorsDayton T. Reardan, Larry J. Smith, Robert E. Klem
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Abstract
Covalently reactive antigen analogs are disclosed herein. The antigens of the invention may be used to stimulate production of catalytic antibodies specific for predetermined antigens associated with particular medical disorders. The antigen analogs may also be used to permanently inactivate endogenously produced catalytic antibodies produced in certain autoimmune diseases as well as in certain lymphoproliferative disorders. Also provided are methods for modulating the efficacy of nucleic acid based therapeutics.
Claims51
A method for modulating the efficacy of a nucleic acid based therapeutic comprising
- a) exposing said therapeutic to an agent under conditions suitable for a chemical reaction with said therapeutic, if any is present;
- b) assessing whether said agent alters the molecular configuration of the nucleic acid based therapeutic or alters the attractive interaction between the therapeutic and the nucleic acid target;
- said method further comprising providing a packaged pharmaceutical preparation of the nucleic acid based therapeutic to be administered wherein the instructions additionally include contraindication and adverse reaction information pertaining to agent(s) identified in step b) in the packaged pharmaceutical composition.
The method as claimed in claim 2, wherein the reaction is a covalent reaction which causes a reduction in the binding energy of said nucleic acid based therapeutic to a target nucleic acid.
A method for modulating the efficacy of a nucleic acid based therapeutic by limiting exposure to an agent capable of alkylating one or more of bases of said therapeutic, said method comprising providing a packaged pharmaceutical preparation of the composition to be administered wherein the instructions additionally include contraindication and adverse reaction information pertaining to the packaged pharmaceutical composition.
A method for modulating the efficacy of a nucleic acid based therapeutic by controlling for exposure to acetaminophen, said method comprising providing a packaged pharmaceutical preparation of the composition to be administered wherein the instructions additionally include contraindication and adverse reaction information pertaining to the packaged Pharmaceutical Composition.
A method for inhibiting the action of a nucleic acid based therapeutic comprising administering an effective amount of acetaminophen to a patient in need thereof, such that reactive intermediates are formed which inactivate said therapeutic.
A method for increasing the efficacy of nucleic acid-based therapy comprising administering a composition comprising a therapeutic amount of a nucleic acid based therapeutic to a human in need thereof, while excluding the administration of acetaminophen to said human so that the nucleic acid molecule and acetaminophen are not present in the human in vivo at the time the nucleic acid based therapeutic is producing its therapeutic effect.
A method of determining the efficacy of a pharmaceutical composition comprising detecting the presence or absence of N-acetyl-p-benzoquinone immune (NAPQI) in a biological sample, wherein the presence of NAPQI correlates with reduced efficacy of the pharmaceutical composition.
A method for identifying biochemical interactions which modulate the efficacy of nucleic acid based therapeutics in vivo comprising:
- a) contacting said therapeutic with an agent which is capable of reacting with said nucleic acid based therapeutic;
- b) assessing whether said agent alters the molecular configuration of the nucleic acid based therapeutic or alters the attractive interaction between the therapeutic and the nucleic acid target, agents so identified which reduce the efficacy of said nucleic acid based therapeutic being contraindicated in patients being treated with said nucleic acid based therapeutic.
Dependencies are inferred from the claim text.