Mu-1, member of the cytokine receptor family
AssigneeGenetics Institute, Inc.
InventorsDebra D. Donaldson, Michelle J. Unger
Life of this patent
Estimating the term…
Abstract
Polynucleotides encoding the MU-1 hematopoietin receptor superfamily chain and fragments thereof are disclosed. MU-1 proteins and methods for their production are also disclosed.
Claims75
1-8. (canceled)
An isolated MU-1 protein comprising an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:2;
- (b) the amino acid sequence of SEQ ID NO:2 from amino acids 22 to 538;
- (c) the amino acid sequence of SEQ ID NO:2 from amino acids 22 to 236;
- and
- (d) the amino acid sequence of SEQ ID NO:2 from amino acids 1 to 236.
A MU-1 protein produced according to the process of
- (A) growing in a suitable culture medium a host cell transformed with a polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:1;
- (b) the nucleotide sequence of SEQ ID NO:1 from nucleotide 238 to nucleotide 1852;
- (c) the nucleotide sequence of SEQ ID NO:1 from nucleotide 301 to nucleotide 1852;
- (d) the nucleotide sequence of SEQ ID NO:1 from nucleotide 301 to nucleotide 945;
- (e) a nucleotide sequence encoding the protein encoded by (b) above in the reading frame beginning with nucleotide 238 and varying from the nucleotide sequence of (b) as a result of degeneracy of the genetic code;
- (f) a nucleotide sequence encoding the protein encoded by (c) above in the reading frame beginning with nucleotide 301 and varying from the nucleotide sequence of (c) as a result of degeneracy of the genetic code;
- and
- (g) a nucleotide sequence encoding the protein encoded by (d) above in the reading frame beginning with nucleotide 301 and varying from the nucleotide sequence of (d) as a result of degeneracy of the genetic code;
- and
- (B) purifying the MU-1 protein from the culture.
16-26. (canceled)
An isolated MU-1 protein which is encoded by a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:1;
- (b) the nucleotide sequence of SEQ ID NO:1 from nucleotide 238 to nucleotide 1852;
- (c) the nucleotide sequence of SEQ ID NO:1 from nucleotide 301 to nucleotide 1852;
- (d) the nucleotide sequence of SEQ ID NO:1 from nucleotide 301 to nucleotide 945;
- (e) a nucleotide sequence encoding the protein encoded by (b) above in the reading frame beginning with nucleotide 238 and varying from the nucleotide sequence of (b) as a result of degeneracy of the genetic code;
- (f) a nucleotide sequence encoding the protein encoded by (c) above in the reading frame beginning with nucleotide 301 and varying from the nucleotide sequence of (c) as a result of degeneracy of the genetic code;
- and
- (g) a nucleotide sequence encoding the protein encoded by (d) above in the reading frame beginning with nucleotide 301 and varying from the nucleotide sequence of (d) as a result of degeneracy of the genetic code.
An isolated soluble fragment of a MU-1 protein comprising at least 90 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:2.
An isolated soluble fragment of a MU-1 protein encoded by a nucleic acid molecule comprising at least 500 contiguous nucleotides of SEQ ID NO:
- 1.
A fusion polypeptide comprising a soluble fragment of a MU-1 protein, wherein the soluble fragment comprises at least 90 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:2 linked to a heterologous polypeptide.
A fusion polypeptide comprising a soluble fragment of a MU-1 protein encoded by a nucleic acid molecule comprising at least 500 contiguous nucleotides of SEQ ID NO:1 linked to a heterologous polypeptide.
An isolated soluble fragment comprising the extracellular domain of a full-length or a mature MU-1 protein of SEQ ID NO:2.
An isolated soluble fragment of a MU-1 protein comprising at least 90 contiguous amino acid residues of an amino acid sequence with at least 80% identity to the amino acid sequence set forth in SEQ ID NO:2.
An isolated soluble fragment of a MU-1 protein encoded by a nucleic acid molecule comprising at least 500 contiguous nucleotides of a nucleotide sequence with at least 80% identity to the nucleotide sequence set forth in SEQ ID NO:1.
Dependencies are inferred from the claim text.