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US 2008/0166805 A1Published application
Culturing human cells and tissues in an N-glycolylneuraminic acid-free environment
AssigneeThe Regents of the University of California
InventorsAjit Varki, Anna Maria Hedlund, Dzung Nguyen
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Abstract
This application is in the field of sialic acid chemistry, metabolism, antigenicity, and the production of transgenic non-human mammals with altered sialic acid production. More particularly, this application relates to N-glycolylneuraminic acid (Neu5Gc) being an immunogen in humans, and the production of Neu5Gc-free mammalian products for laboratory and human use.
Claims9
A method for preparing a culture of human cells devoid of N-glycolylneuraminic acid (Neu5Gc) comprising the steps of:
- preselecting the human cells to be cultured;
- placing the human cells in a culture medium comprising serum selected from the group consisting of:
- animal-free serum replacement, mammalian-free serum, human serum, heat-inactivated human serum, and Neu5Gc deficient animal serum;
- and
- allowing the human cells to grow in the culture medium to a desired density.
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