Trimerizing Polypeptides and Their Uses
AssigneeBarnes-Jewish Hospital
InventorsAudrey McAlinden, Erika Crouch, Linda Sandell
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Abstract
A method for trimerizing collagenous molecule monomers comprising the step of contacting a collagen domain and a non-collagenous trimerization domain is provided. In addition, methods of trimerizing heterologous peptides is provided. Trimerizing polypeptides, vectors, cells, and trimerized polypeptides are also provided.
Claims23
1-35. (canceled)
A method for trimerizing polypeptides, the method comprising:
- (a) providing at least three polypeptides comprising a collagenous domain linked to a non-collagenous trimerization domain, said trimerization domain having the formula
- (abcdefg)n
- wherein positions a and d comprise hydrophobic residues;
- positions e and g comprise charged residues;
- positions b, c and f comprise polar or charged residues;
- n is 2 or 3;
- and
- (b) contacting the polypeptides.
A polypeptide trimer comprising three polypeptides comprising a collagenous domain linked to a non-collagenous trimerization domain, said trimerization domain having the formula
- (abcdefg)n
- wherein positions a and d comprise hydrophobic residues;
- positions e and g comprise charged residues;
- positions b, c and f comprise polar or charged residues;
- and n is 2 or 3.
A polypeptide comprising a collagenous domain linked to a non-collagenous trimerization domain, said trimerization domain having the formula
- (abcdefg)n
- wherein positions a and d comprise hydrophobic residues;
- positions e and g comprise charged residues;
- positions b, c and f comprise polar or charged residues;
- and n is 2 or 3.
Dependencies are inferred from the claim text.