Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
AssigneeThe Regents of the University of California
InventorsNeil R. Beer, Benjamin J. Hindson, Billy W. Colson, Joseph P. Fitch
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Abstract
A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
Claims28
An apparatus for identifying the known and unknown pathogenic or non-pathogenic organisms in a sample wherein the organisms include nucleic acids, comprising:
- a droplet generator for creating droplets from the sample wherein said droplets constitute sub-nanoliter volume reactors containing said organism sized particles;
- a lysis device for performing lysis of the organisms to release the nucleic acids;
- an amplifier for amplifying the nucleic acids;
- a fractionater that releases the nucleic acids from said droplets;
- and
- a parallel analyzer for identifying all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
An apparatus for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample wherein the organisms include nucleic acids, comprising:
- a chip,
- a microfluidic flow channel in said chip,
- a droplet generator in said microfluidic flow channel for creating droplets from the sample wherein said droplets constitute sub-nanoliter volume reactors containing said organism sized particles;
- a lysis device in said microfluidic flow channel for performing lysis of the organisms to release the nucleic acids;
- an amplifier in said microfluidic flow channel for amplifying the nucleic acids;
- a fractionater in said microfluidic flow channel that releases the nucleic acids from said droplets;
- and
- a parallel analyzer connected to said microfluidic flow channel for identifying all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
Dependencies are inferred from the claim text.