Polynucleotides allowing the expression and secretion of recombinant HBsAg virus-like particles containing a foreign peptide, their production and use
OwnerMonica Sala-Schaeffer, Marie Michel +1inventors
InventorsMonica Sala-Schaeffer, Marie Michel, Simon Wain-Hobson
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Abstract
The hepatitis B surface antigen (HBsAg) can assemble into sub-virion virus like particles (VLPs). Vectors comprised of polynucleotides, identified as GA1xFlag-M and GA3xFlag-M, are provided for the expression of foreign proteins in such VLPs, and for release of the VLPs from host cells containing the vectors. In one example, an HIV-1 polyepitope-HBsAg recombinant fusion protein assembled into VLPs and was efficiently secreted.
Claims19
A polynucleotide comprising GA1xFlag-M polynucleotide of sequence:
- [SEQ ID NO:
- 1]
- CAGGCCATGCAGTGGAACTCCACAcccgggGCTGGAGCAGGAGCT
- GATTACAAGGACGACGACGACAAGgaattcCTGCAGGCTAGCAGA
- TCTctcgagCTGAACATG;
- or GA3XFlag-M polynucleotide of sequence:
- [SEQ ID NO:
- 2]
- CAGGCCATGCAGTGGAACTCCACAcccgggGCTGGAGCAGGAGCT
- GACTACAAAGACCACGACGGTGATTATAAAGATCACGACATTGAT
- TACAAGGACGACGACGACAAGgaattcCTGCAGGCTAGCAGATCT
- ctcgagCTGAACATG.
A polynucleotide as claimed in any of claims 1 to 10, wherein it further comprises a foreign coding polynucleotide inserted in any of restriction sites of the GA1xFlag-M or GA3xFlag-M polynucleotide and in frame with the ATG at position 7 in the GA1xFlag-M or GA3xFlag-M polynucleotide sequence.
Dependencies are inferred from the claim text.