Abasic site endonuclease assay
AssigneeEpoch Biosciences, Inc.
InventorsIgor V. Kutyavin, David Milesi, Merl F. Hoekstra
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Abstract
The present invention provides a novel method for detection and/or genotyping of nucleic acids that utilizes the specificity of an AP endonuclease. In addition, the present invention provides a novel method for nucleic acid amplification.
Claims28
A kit comprising an AP site probe comprising an oligonucleotide NA that hybridizes to a target nucleic acid, a quencher molecule attached to the NA, and a functional tail R comprising a detectable reporter group, said functional tail R attached via a phosphodiester bond of a phosphate group to the 3′ terminal nucleotide of the NA, wherein the reporter group is not detected when the functional tail R is attached to the NA.
Dependencies are inferred from the claim text.