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US 2008/0166778 A1Published application
Method of organic polymer degradation by dihydrolipoamide dehydrogenase
AssigneeNational Central University
InventorsShir-Ly Huang, Yu-Ling Sun
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Abstract
A dihydrolipoamide dehydrogenase (DLD) in a germ is recombined. The new DLD is applied in a solution to degrade an ether bond of an organic polymer. With the present invention, bioremediation is accomplished without secondary pollution of compounds which have environmental hormones.
Claims11
A method of an organic polymer decomposition by using dihydrolipoamide dehydrogenase (DLD), comprising steps of:
- (a) obtaining a gene of DLD from a protein;
- (b) recombining a fusion protein of said DLD in Escherichia coli to obtain a recombinant deoxyribonucleic acid (DNA);
- (c) processing an isolation and a purification of said recombinant DLD through two steps of column chromatography by affinity and gel filtration;
- (d) obtaining a solution having said DLD and processing an analysis of said DLD in said solution by using a mass spectrometer, wherein an ether bond of an organic polymer is degraded.
1 more element
The method according to claim 10,
- wherein said recombined DNA is obtained through transcribing a gene of said protein of Pseudomonas nitroreducens TX1;
- and
- wherein said gene is obtained from DLD of said Pseudomonas nitroreducens TX1.
Dependencies are inferred from the claim text.