Methods of increasing the concentration of a nucleic acid
OwnerPhillip Hollinger, Farid Ghadassy +1inventors
InventorsPhillip Hollinger, Farid Ghadassy, Jennifer Lee Ong
Life of this patent
Estimating the term…
Abstract
We describe a method of selecting an enzyme having replicase activity, the method comprising the steps of: (a) providing a pool of nucleic acids comprising members each encoding a replicase or a variant of the replicase; (b) subdividing the pool of nucleic acids into compartments, such that each compartment comprises a nucleic acid member of the pool together with the replicase or variant encoded by the nucleic acid member; (c) allowing nucleic acid replication to occur; and (d) detecting amplification of the nucleic acid member by the replicase. Methods for selecting agents capable of modulating replicase activity, and for selecting interacting polypeptides are also disclosed.
Claims20
(canceled)
A method for increasing concentration of a nucleic acid molecule comprising the steps of:
- (a) forming microcapsules from a thermostable water-in-oil emulsion, wherein a plurality of microcapsules include a nucleic acid molecule, a bead capable of being linked to the nucleic acid molecule, and an aqueous solution comprising components necessary to perform PCR nucleic acid amplification, including a thermostable polymerase, wherein the emulsion has a density of greater than 109 microcapsules per ml;
- (b) amplifying the nucleic acid molecule in the microcapsules to form amplified copies of the nucleic acid molecule;
- thereby increasing the concentration of the nucleic acid molecule;
- and
- (c) sorting the bead with the captured amplification copies for sequencing.
Dependencies are inferred from the claim text.