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OwnerTadayuki Uno, Yohei Hoashi +2inventors
InventorsTadayuki Uno, Yohei Hoashi, Naohisa Katayama, Toshiya Kai
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Provided is a high-copy-number, high-expression vector capable of producing a protein having satisfactory functions and activity of the same level as that of a natural form of the protein, in a large quantity and in a simple manner. Also provided is a vector including: (A) a target gene or a cloning site of the target gene, (B) a sequence element necessary for the high copying of the target gene, (C) a sequence element necessary for the expression of the target gene and a methionine aminopeptidase gene; a method of producing the vector; a transformant having the vector introduced therein; and a process for producing a protein using the transformant.
A vector comprising (A) a target gene or a cloning site where the target gene is inserted, (B) a sequence element necessary for high copying of the target gene, (C) a sequence element necessary for expression of the target gene, and (D) a methionine aminopeptidase gene.
A method of producing a vector, comprising the steps of
A transformant introduced with a vector comprising (A) a target gene, (B) a sequence element necessary for high copying of the target gene, (C) a sequence element necessary for expression of the target gene, and (D) a methionine aminopeptidase gene.
A method of producing a protein, comprising cultivating in a culture medium a transformant introduced with a vector comprising (A) a target gene, (B) a sequence element necessary for high copying of the target gene, (C) a sequence element necessary for expression of the target gene, and (D) a methionine aminopeptidase gene, and
Dependencies are inferred from the claim text.