Dna construct and process for the fermentative production of fusion proteins
AssigneeWacker Chemie AG
InventorsThomas Schloesser, Tobias Dassler, Kerstin Pfeiffer-Schwiesow
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Abstract
A DNA construct that allows the inexpensive production of a target protein in E. coli, consisting of a nucleic acid sequence encoding a signal peptide which is operably linked with a gene coding for a carrier protein which is linked with a gene coding for the target protein via a gene coding for a cleavable sequence S, wherein the gene coding for a carrier protein is the spy gene from E. coli.
Claims18
A DNA construct for producing a target protein in E. coli, the DNA construct comprising a nucleic acid sequence encoding a signal peptide which is operably linked with a gene coding for a carrier protein which is linked with a gene coding for the target protein via a gene coding for a cleavable sequence S, wherein the gene coding for a carrier protein is a spy gene from E. coli.
10 A method of generating a fusion-protein-secreting microorganism strain, wherein the DNA construct of claim 1 is introduced into a microorganism strain from the family Enterobacteriaceae.
11 A method of generating a fusion-protein-secreting microorganism strain, wherein the plasmid of claim 9 is introduced into a microorganism strain from the family Enterobacteriaceae.
A fusion protein comprising a Spy protein, a target protein, and a cleavable sequence (S).
A carrier protein for the expression of a target protein in E. coli, the carrier protein comprising a Spy protein from E. coli.
Dependencies are inferred from the claim text.